TIMP1 ELISA Kit

Produktnummer ABIN625099

Produktdetails TIMP1 ELISA Kit

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 30-18.000 pg/mL

Untere Nachweisgrenze: 30 pg/mL

Applikation: ELISA

Verwendungszweck: Human TIMP-1 ELISA Kit for cell and tissue lysate samples.

Proben: Tissue Lysate, Cell Lysate

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes human TIMP-1.

Sensitivität: 30 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis
• Cell lysate buffer

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Andere Bezeichnung: TIMP-1 (TIMP1 Produkte)

Synonyme: TIMP-1 ELISA Kit, TIMP1 ELISA Kit, DKFZp468A0912 ELISA Kit, CLGI ELISA Kit, EPA ELISA Kit, EPO ELISA Kit, HCI ELISA Kit, TIMP ELISA Kit, Timp ELISA Kit, Clgi ELISA Kit, TIMP metallopeptidase inhibitor 1 ELISA Kit, tissue inhibitor of metalloproteinase 1 ELISA Kit, TIMP1 ELISA Kit, Timp1 ELISA Kit

Hintergrund: Gene Names: TIMP1 CLGI TIMP
Protein names: Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)

Gen-ID: 7076

UniProt: P01033