MMP8 ELISA Kit
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- Target Alle MMP8 ELISA Kits anzeigen
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 6-6000 pg/mL
- Untere Nachweisgrenze
- 6 pg/mL
- Applikation
- ELISA
- Verwendungszweck
- Human MMP-8 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
- Sensitivität
- 6 pg/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product MMP8 ELISA Kit
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 - 20 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Aufbereitung der Proben
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Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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-
Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).
: "
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Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).
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- Target Alle MMP8 ELISA Kits anzeigen
- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
- Andere Bezeichnung
- MMP-8 (MMP8 Produkte)
- Synonyme
- MMP8 ELISA Kit, BB138268 ELISA Kit, CLG1 ELISA Kit, HNC ELISA Kit, MMP-8 ELISA Kit, PMNL-CL ELISA Kit, matrix metallopeptidase 8 ELISA Kit, neutrophil collagenase ELISA Kit, MMP8 ELISA Kit, Mmp8 ELISA Kit, LOC100339790 ELISA Kit
- Hintergrund
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Gene Names: MMP8 CLG1
Protein names: Neutrophil collagenase (EC 3.4.24.34) (Matrix metalloproteinase-8) (MMP-8) (PMNL collagenase) (PMNL-CL)
- Gen-ID
- 4317
- UniProt
- P22894
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