USP20 ELISA Kit

Produktnummer ABIN6222185

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human USP20.

Kurzübersicht für USP20 ELISA Kit (ABIN6222185)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reaktivität: Human

Nachweismethode: Colorimetric

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Produktdetails USP20 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human Ubiquitin Carboxyl-Terminal Hydrolase 20 (USP20) ELISA Kit

Analytische Methode: Quantitative

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Applikationshinweise: Optimal dilutions/concentrations should be determined by the end user.

Kommentare:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.

• 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.

• 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

Probennahme:

• Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4 °C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 x g for 20 min. Analyze the serum immediately or aliquot and store at -20 °C or -80 °C.

• Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediately or aliquot and store at -20 °C or -80 °C. Avoid hemolysis and high cholesterol samples.

• Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type - this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 x g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20 °C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Haltbarkeit: 6 months

Details zu USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Andere Bezeichnung: Ubiquitin Carboxyl-Terminal Hydrolase 20

Hintergrund:

Ensembl: ENSG00000136878

UniProt Entry Name: UBP20_HUMAN

Gen-ID: 10868

HGNC: 12619

OMIM: 615143

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling