beta-2 Microglobulin ELISA Kit

Produktnummer ABIN577106

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human beta-2 Microglobulin.

Kurzübersicht für beta-2 Microglobulin ELISA Kit (ABIN577106)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktdetails beta-2 Microglobulin ELISA Kit

Verwendungszweck: This B2MG enzyme linked immunosorbent assay (ELISA) kit uses a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for B2MG. Standards or samples are added to the microtiter plate wells and B2MG, if present, will bind to the antibody pre-coated on the wells. A preparation of horseradish peroxidase (HRP)-conjugated B2MG antibody is added to each well. The conjugated antibody will bind to the B2MG immobilized on the plate after incubation. All unbound components will be removed by subsequent washing procedure. Next, 3, 3', 5, 5' tetramethyl-benzidine(TMB), a substrate for HRP is added to each well. The colorimetric reaction is proportional to the B2MG content in each well. The reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured p s ectrophotometrically at the wavelength of 450nm 2nm. In order to quantify the amount of B2MG present in the sample, this Human B2MG ELISA Kit includes a set of calibration standards (6 standards). The calibration standards should be assayed at the same time as the samples to allow the operator to produce a standard curve of Optical Density (O.D.) versus B2MG concentration ( g/mL). The concentration of B2MG in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(03)B2MG 2

Analytische Methode: Quantitative

Sensitivität: S7.5(03)B2MG 8 _x000C_ The minimal detectable dose of this B2MG assay was calculated by adding two standard deviations to the mean optical density value of 16 zero standard replicates and determining the corresponding concentration from the standard curve. The minimal detectable dose for this human B2MG assay generated by this method is 0.2 g/mL.

Bestandteile: Standards: 1 set/2 vials

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Without preservative

Details zu beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung: Beta2-Microglobulin

Hintergrund: Interleukin-11, also known as adipogenesis inhibitory factor (AGIF) and oprelvekin, is a member of the GP13 family of cytokines. These cytokines drive the assembly of multi- subunit receptor complexes containing at least one GP13. IL-11 exerts biological activity through IL-11 receptor by utilizing the gp13 molecule as the signaling component of its receptor. Interleukin 11 (IL-11) is a highly conserved precursor protein of 19kDa with 199 amino acids. The first 21 amino acids are a typical leader sequence for secreted proteins. When the 178 amino acid protein is released from the cell, the first 21 amino acids are removed. IL-11 is thermally stable and contains no cysteine residues. Many cell and tissue types throughout the body, such as CNS, thymus, lung, bone, connective tissues, uterus, skin, and testis, can produce IL-11 depending on the local environment of the cell or tissue. IL11 promotes primary and secondary immune responses in vitro and in vivo and modulates antigen-specific antibody reactions. IL-11 has activity in bone metabolism and activity in protection and restoration of the gastrointestinal mucosa. Human endometrial stromal cells produce biologically active IL-11, which promotes progesterone- induced decidualization. Therefore, the IL-11 has both paracrine and autocrine actions on human endometrial stromal cells and plays an important role in preparing the human endometrium for implantation. Reports indicate that abnormal IL-11 levels in serum or plasma is related to immune thrombocytopenia, rheumatoid arthritis, and urogenital infection. Immunoreactive IL-11 has also been linked to aseptic loosening of total hip replacement implants. The production of IL-11 is elevated in lesional skin organ cultures of patients with active plaque-type psoriasis as compared with non-lesional and normal skin. The human IL-11 genes contain five exons, each having a length of 7 kDa, and are mapped to chromosome 19q13. 3-13. 4. Primate and human IL-11 sequences show 97 percent homology. The human IL-11 receptor gene maps to chromosome 9p13. This IL-11 ELISA Kit is a ready-to-use 3.5-hour solid phase immunoassay capable of measuring IL-11 levels in serum, plasma, cell culture supernatant in the range of to 16 pg/mL. This kit has been shown to have a specific reaction with IL-11 without any cross reactivity with various other cytokine super-family proteins. S7.5(5) IL-11 2

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process