Ultrasensitive thyroid stimulating hormone ELISA Kit

Produktnummer ABIN577074

Produktdetails

Target: Ultrasensitive Thyroid Stimulating Hormone (uTSH)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: This ultra-sensitive TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody pre- coated on the wells. In order to quantitate the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to

Analytische Methode: Quantitative

Sensitivität: The minimal detectable concentration of TSH by this assay is estimated to be 0.05 IU/mL.

Bestandteile: Standards: 1 set/2 vials

Anwendungsinformationen

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Without preservative

Antigendetails

Target: Ultrasensitive Thyroid Stimulating Hormone (uTSH)

Hintergrund: Interleukin-8 (IL-8), also known as neutrophil attractant/activating protein (NAP-1), monocyte-derived neutrophil-activating peptide (MONAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF) and leukocyte adhesion inhibitor (LAI), is a member of the chemokine superfamily which selectively chemoattract and activate specific leukocyte subpopulations (1,2). All of these cytokines have four conserved cysteines and two distinguishable subfamilies. These two subfamilies are dependent on the position of the first two cysteines, which are either separated by on amino acid (C-X-C proteins) or are adjacent (CC-protein) to each other. The members of the two subfamilies differ in their target cell selectivity as well as the chromosomal location of their genes (chromosome 4 for the C-X-C proteins and chromosome 17 for the C-C proteins). IL-8 belongs to the C-X-C subfamily along with platelet factor 4 (PF4), platelet basic protein (PBP), connective-tissue-activating peptide III (CTAPIII), -thromboglobulin, neutrophil-activating peptide-2 (NAP-2), ENA-78 (3), three closely related MGSA/CRO gene products (GRO- , GRO- , GRO- ), and -interferon-inducible protein ( -IP-1)(4). The members of the C-C chemokines are mainly chemotactic for monocytes whereas the C-X-C chemokines except for IP1 and PF4, chemoattract and activate neutrophils. In addition to the effect on neutrophils, IL-8 has been reported to be a less potent chemoattractant for T lymphocytes (5). IL-8 is produced by many cells in response to inflammatory stimuli such as IL-1 or TNF- and to various types of mitogen, lectins, crystals, viruses, and phorbol esters (PMA). Many cell types that produce IL-8 in response to these stimuli can include: monocytes/ macrophages, T lymphocytes, neutrophils, fibroblasts, keratinocytes, hepacytes, chondrocytes, endothelial cells, glioblatoma cells , and mesothelial cells (6). The IL-8 predominant form secreted by stimulated monocytes has 72 residues (MW=8385), whereas the predominant form secreted by IL-1 stimulated endothelial cells has 77 residues (MW=8922). These variants have similar biological activities, although the 72-residue form of IL-8 appears to be 2 to 1 fold more potent than the 77-residue form depending on the type of assay used (7). Various non-infectious human diseases are known to be associated with neutrophilia and/or neutrophil infiltration into organs. Examples of some of these human diseases include rheumatoid arthritis, gouty arthritis, psoriasis, glomerulonephritis, adult respiratory distress syndrome, immune vasculitis, inflammatory bowel disease, ischemia-reperfusion syndrome (including myocardial infarction and multiple organ failure), chorioretinitis, cystic fibrosis, S7.5(3) IL-8 _x000C_ septic shock, acute meningococcal infections, alcoholic hepatitis and mediterranean fever (8). The presence of IL-8 has been positively identified in gouty arthritis, psoriatic scale, plasma from adult respiratory syndrome caused by sepsis, and serum from nephrotic syndrome as well as in the joint fluids from rheumatoid arthritis. The peripheral blood mononuclear cells (PBMC) obtained from patients undergoing an asthmatic attack have been shown to spontaneously produce in vitro IL-8-like molecules. The production of IL-8 triggers many other activities that contribute to these human diseases; however, IL-8 is not known to trigger systemic inflammatory reactions such as fever, acute phase protein induction. Development of an accurate immunoassay for the quantitative determination of human IL-8 levels in cell culture supernatant, serum, plasma and other biological fluids is expected to be effectively used for the further investigation of the relationship between IL-8 and various inflammatory diseases. This IL-8 ELISA is a 2.5 hour solid-phase immunoassay readily applicable to measure IL-8 levels in serum, plasma, cell culture supernatant, and other biological fluids ranging from to 16 pg/mL. It has shown no cross-reactivity with human monocyte chemotactic activating factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). This IL-8 ELISA is expected to be effectively used for further investigations into the relationship between IL-8 and various diseases. PRINCIPLES OF THE ASSAY This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8 if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of the sample. In order to quantify the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for IL-8 is added to each well to