Total Bile Acid (TBA) Colorimetric Assay Kit

Produktnummer ABIN7121144

Kurzübersicht für Total Bile Acid (TBA) Colorimetric Assay Kit (ABIN7121144)

Target: Total Bile Acide

Nachweismethode: Colorimetric

Applikation: Biochemical Assay (BCA)

Proben: Serum, Tissue Samples

Analytische Methode: Quantitative

Produktdetails

Detektionsbereich: 1.77 μmol/L - 40 μmol/L

Untere Nachweisgrenze: 1.77 μmol/L

Verwendungszweck: The Total Bile Acid (TBA) Assay Kit is biochemical kit in Colorimetric method,can be used for in vitro quantitative determination of total bile acids content in serum.

Sensitivität: 1.36 μmol/L

Produktmerkmale: With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

Benötigtes Material: Spectrophotometry (Visible range), Biochemistry analyzer

Anwendungsinformationen

Testdauer: 25 min

Testpräzision:

Inter-assay precision: 3.7%

Intra-assay precision: 3.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Protect from light.

Lagerung: 4 °C

Informationen zur Lagerung: This product can be stored at 2~8°C for 12 months with shading light.

Haltbarkeit: 12 months

Antigendetails

Target: Total Bile Acide

Andere Bezeichnung: Total Bile Acid