Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)
Kurzübersicht für Glycogen Colorimetric Assay Kit (Liver/Muscle Samples) (ABIN5708882)
Target
Nachweismethode
Applikation
Proben
Analytische Methode
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Detektionsbereich
- 1.8 mg/g - 180 mg/g
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Untere Nachweisgrenze
- 1.8 mg/g
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Verwendungszweck
- This kit can be used for detection of glycogen content in animal muscle, liver and other samples.
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Produktmerkmale
- Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
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Benötigtes Material
- Spectrophotometry (Visible range)
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Testdauer
- 1 h
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Testpräzision
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Inter-assay precision: 7.3%
Intra-assay precision: 3.7%
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Protect from light.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- This product can be stored at 2~8°C for 12 months with shading light.
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Haltbarkeit
- 12 months
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- Glycogen
Target
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