Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Kurzübersicht für Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (ABIN5708826)
Target
Alle ALT Kits anzeigenNachweismethode
Applikation
Proben
Analytische Methode
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Detektionsbereich
- 1.26 IU/L - 72.3 IU/L
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Untere Nachweisgrenze
- 1.26 IU/L
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Verwendungszweck
- The Alanine Aminotransferase (ALT/GPT) Assay Kit (Reitman-Frankel method) is biochemical kit in Colorimetric method
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Produktmerkmale
- ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37 °C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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Benötigtes Material
- Spectrophotometry (Visible range)
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Testdauer
- 1.5 h
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Testpräzision
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Inter-assay precision: 8.2%
Intra-assay precision: 4.3%
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Protect from light.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- This product can be stored at 2~8°C for 12 months with shading light.
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Haltbarkeit
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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Andere Bezeichnung
- ALT
Target
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