MYOD1 ELISA Kit

Produktnummer ABIN5706920

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus MYOD1.

Kurzübersicht für MYOD1 ELISA Kit (ABIN5706920)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.1 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails MYOD1 ELISA Kit

Untere Nachweisgrenze: 78.1 pg/mL

Analytische Methode: Quantitative

Sensitivität: 29 pg/mL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Probenmenge: 100 µL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: Myogenic Differentiation (MyoD)

Hintergrund: Alternative name: MYOD1, PUM, MYF3, bHLHc1, Myoblast Determination Protein 1, Myogenic Factor 3, Class C basic helix-loop-helix protein 1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development