RALBP1 ELISA Kit

Produktnummer ABIN5706907

Ratte RALBP1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte RALBP1.

Kurzübersicht für RALBP1 ELISA Kit (ABIN5706907)

Target: RALBP1 (RalA Binding Protein 1 (RALBP1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails RALBP1 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Analytische Methode: Quantitative

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Probenmenge: 100 µL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu RALBP1

Target: RALBP1 (RalA Binding Protein 1 (RALBP1))

Hintergrund: Alternative name: RIP, RIP1, RLIP76, 76 kDa Ral-interacting protein, Ral-interacting protein 1, Dinitrophenyl S-glutathione ATPase