Myosin X ELISA Kit

Produktnummer ABIN5706904

Human Myosin X ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Myosin X.

Kurzübersicht für Myosin X ELISA Kit (ABIN5706904)

Target: Myosin X (MYO10)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails Myosin X ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.114 ng/mL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Probenmenge: 50 µL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Myosin X

Target: Myosin X (MYO10)

Hintergrund: Alternative name: Unconventional Myosin-X, Unconventional Myosin-10