BMP15 ELISA Kit

Produktnummer ABIN5706899

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Schwein BMP15.

Kurzübersicht für BMP15 ELISA Kit (ABIN5706899)

Target: BMP15 (Bone Morphogenetic Protein 15 (BMP15))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails BMP15 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Analytische Methode: Quantitative

Sensitivität: 10.4 pg/mL

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Probenmenge: 100 µL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu BMP15

Target: BMP15 (Bone Morphogenetic Protein 15 (BMP15))

Hintergrund: Alternative name: GDF9B, GDF9-B, ODG2, POF4, Growth/differentiation factor 9B