LALBA ELISA Kit

Produktnummer ABIN5696562

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Rind (Kuh) LALBA.

Kurzübersicht für LALBA ELISA Kit (ABIN5696562)

Target: LALBA (Lactalbumin, alpha- (LALBA))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 3.125 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails LALBA ELISA Kit

Untere Nachweisgrenze: 3.125 ng/mL

Verwendungszweck: Bovine LALBA (Alpha-lactalbumin) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with LALBA , no obvious cross reaction with other analogues.

Sensitivität: 1.875 ng/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP-Antigen(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antibody

Testdauer: 1 h

Plattentyp: Pre-coated

Protokoll: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. (Equilibrate the whole bottle of TMB for 30 min at 37 °C.) Washing: Wash the plate five times and immerse for 1 min each time. Step 3: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu LALBA

Target: LALBA (Lactalbumin, alpha- (LALBA))

Andere Bezeichnung: LALBA (Alpha-lactalbumin)

Hintergrund: Alpha-lactalbumin, Lactose synthase B protein, Lysozyme-like protein 7, LALBA, LYZL7