Anti Deoxyribonucleic Acid Antibody ELISA Kit

Produktnummer ABIN5696338

Das Maus Anti Deoxyribonucleic Acid Antibody ELISA Kit (ABIN5696338) ist ein Colorimetric ELISA Kit zur Detektion von Maus Anti Deoxyribonucleic Acid Antibody.

Kurzübersicht für Anti Deoxyribonucleic Acid Antibody ELISA Kit (ABIN5696338)

Target: Anti Deoxyribonucleic Acid Antibody

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Detektionsbereich: 0.313 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails

Untere Nachweisgrenze: 0.313 ng/mL

Verwendungszweck: Mouse Anti-dsDNA (double stranded DNA) antibody ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with dsDNA-Ab , no obvious cross reaction with other analogues.

Sensitivität: 1.875 ng/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP Antibody(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Indirect ELISA

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Antigendetails

Target: Anti Deoxyribonucleic Acid Antibody

Andere Bezeichnung: Anti-DNA antibody

Substanzklasse: Antibody

Hintergrund: DsDNA antibody, double stranded DNA antibody