HYAL1 ELISA Kit

Produktnummer ABIN5692203

Human HYAL1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human HYAL1.

Kurzübersicht für HYAL1 ELISA Kit (ABIN5692203)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Bindungsspezifität: AA 22-435

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA - heparin), Serum

Produktdetails HYAL1 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Hyaluronidase1/HYAL1. 96wells/kit, with removable strips.

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO, Immunogen sequence: F22-W435

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Anwendungsinformationen

Testdauer: 15 min

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Haltbarkeit: 12 months

Details zu HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Andere Bezeichnung: HYAL1

Hintergrund:

Synonyms: Hyaluronidase-1, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1, HYAL1, LUCA1

Tissue Specificity: Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.

Background: Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic  pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Cellular Localisation: Secreted

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process