Peroxiredoxin 5 ELISA Kit
Kurzübersicht für Peroxiredoxin 5 ELISA Kit (ABIN5690930)
Target
Alle Peroxiredoxin 5 (PRDX5) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
-
-
Verwendungszweck
- Human Peroxiredoxin-5 ELISA Kit.
-
Analytische Methode
- Quantitative
-
Spezifität
- This ELISA antibody pair recognizes Human Peroxiredoxin-5.
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
-
Plattentyp
- Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- 4 °C
-
Haltbarkeit
- 6 months
-
-
- Peroxiredoxin 5 (PRDX5)
-
Andere Bezeichnung
- Peroxiredoxin-5
-
Gen-ID
- 25824
-
UniProt
- P30044
-
Pathways
- Cell RedoxHomeostasis
Target Alle Peroxiredoxin 5 (PRDX5) ELISA Kits anzeigen
-