Neurogenin 3 ELISA Kit

Produktnummer ABIN5690917

Produktdetails Neurogenin 3 ELISA Kit

Target: Neurogenin 3 (NEUROG3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Neurogenin-3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Neurogenin-3.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Andere Bezeichnung: Neurogenin-3 (NEUROG3 Produkte)

Synonyme: NEUROG3 ELISA Kit, ngn3 ELISA Kit, atoh5 ELISA Kit, ngn-3 ELISA Kit, bhlha7 ELISA Kit, math4b ELISA Kit, neurog3 ELISA Kit, Ngn3 ELISA Kit, Atoh5 ELISA Kit, Math4B ELISA Kit, NGN-3 ELISA Kit, bHLHa7 ELISA Kit, Relax ELISA Kit, atoh4 ELISA Kit, ngn2 ELISA Kit, ngn3a ELISA Kit, neurogenin 3 ELISA Kit, neurogenin 3 S homeolog ELISA Kit, neurogenin-3 ELISA Kit, NEUROG3 ELISA Kit, neurog3 ELISA Kit, neurog3.S ELISA Kit, Tsp_02556 ELISA Kit, Neurog3 ELISA Kit

Gen-ID: 50674

UniProt: Q9Y4Z2