FUT11 ELISA Kit
Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human FUT11.
Produktnummer ABIN5690878
Kurzübersicht für FUT11 ELISA Kit (ABIN5690878)
Target
Alle FUT11 ELISA Kits anzeigen
FUT11
(Fucosyltransferase 11 (Alpha (1,3) Fucosyltransferase) (FUT11))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Verwendungszweck
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Human FUT11 ELISA Kit.
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Human FUT11.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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FUT11
(Fucosyltransferase 11 (Alpha (1,3) Fucosyltransferase) (FUT11))
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Andere Bezeichnung
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FUT11
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Gen-ID
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170384
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UniProt
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Q495W5
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