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DIDO1 ELISA Kit

DIDO1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690865
$728.20
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 31 bis 41 Werktagen
  • Target Alle DIDO1 ELISA Kits anzeigen
    DIDO1 (Death Inducer-Obliterator 1 (DIDO1))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human DIDO1/DIO-1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human DIDO1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften DIDO1 ELISA Kit
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle DIDO1 ELISA Kits anzeigen
    DIDO1 (Death Inducer-Obliterator 1 (DIDO1))
    Andere Bezeichnung
    DIDO1 (DIO-1 (DIDO1 Produkte)
    Synonyme
    5DI, TXDI1, BYE1, C20orf158, DATF-1, DATF1, DIDO2, DIDO3, DIO-1, DIO1, dJ885L7.8, 6720461J16Rik, C530043I07, D130048F08Rik, Datf1, Dido2, Dido3, mKIAA0333, DIDO1, datf1l, fi35b04, wu:fi35b04, zgc:158157, DKFZp469J1523, bye1, dio1, datf1, dido2, dido3, dio-1, iodothyronine deiodinase 1, death inducer-obliterator 1, death inducer-obliterator 1 L homeolog, DIO1, DIDO1, Dido1, dido1.L, dido1
    Gen-ID
    11083
    UniProt
    Q9BTC0
    Pathways
    Hormone Activity
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