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DIDO1 ELISA Kit
Human DIDO1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human DIDO1 in Cell Culture Supernatant, Cell Samples, Plasma, Serum und Tissue Lysate
Produktnummer ABIN5690865
Kurzübersicht für DIDO1 ELISA Kit (ABIN5690865)
Target
Alle DIDO1 ELISA Kits anzeigen
DIDO1
(Death Inducer-Obliterator 1 (DIDO1))
Reaktivität
Alle Reaktivitäten für DIDO1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktdetails DIDO1 ELISA Kit
(ausblenden)
Verwendungszweck
Human DIDO1/DIO-1 ELISA Kit.
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human DIDO1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
4 °C
Haltbarkeit
6 months
Details zu DIDO1
(ausblenden)
DIDO1
(Death Inducer-Obliterator 1 (DIDO1))
Andere Bezeichnung
DIDO1 (DIO-1
Gen-ID
11083
UniProt
Q9BTC0
Pathways
Hormone Activity
Zuletzt angesehen
(ausblenden)
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