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CDK5R1 ELISA Kit

CDK5R1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690848
  • Target Alle CDK5R1 ELISA Kits anzeigen
    CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))
    Reaktivität
    • 3
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CDK5 Activator 1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human CDK5 Activator 1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle CDK5R1 ELISA Kits anzeigen
    CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))
    Andere Bezeichnung
    CDK5 Activator 1 (CDK5R1 Produkte)
    Synonyme
    CDK5P35 ELISA Kit, CDK5R ELISA Kit, NCK5A ELISA Kit, p23 ELISA Kit, p25 ELISA Kit, p35 ELISA Kit, p35nck5a ELISA Kit, cdk5r1 ELISA Kit, zgc:92814 ELISA Kit, CDK5R1 ELISA Kit, Cdk5r ELISA Kit, P25/P35 ELISA Kit, D11Bwg0379e ELISA Kit, Xp35.1 ELISA Kit, cyclin dependent kinase 5 regulatory subunit 1 ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1b (p35) ELISA Kit, cyclin-dependent kinase 5 regulatory subunit 1 ELISA Kit, S100 calcium binding protein A9 ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1 (p35) ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1 (p35) L homeolog ELISA Kit, CDK5R1 ELISA Kit, cdk5r1b ELISA Kit, Cdk5r1 ELISA Kit, S100A9 ELISA Kit, cdk5r1.L ELISA Kit
    Substanzklasse
    Viral Protein
    Gen-ID
    8851
    UniProt
    Q15078
    Pathways
    Stem Cell Maintenance, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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