Catalase ELISA Kit

Produktnummer ABIN5690839

Produktdetails Catalase ELISA Kit

Target: Catalase (CAT)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Catalase ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Catalase.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu Catalase

Target: Catalase (CAT)

Andere Bezeichnung: Catalase (CAT Produkte)

Synonyme: 2210418N07 ELISA Kit, Cas-1 ELISA Kit, Cas1 ELISA Kit, Cs-1 ELISA Kit, CS1 ELISA Kit, Cat01 ELISA Kit, Catl ELISA Kit, fb68a12 ELISA Kit, wu:fb68a12 ELISA Kit, CAT ELISA Kit, CATA ELISA Kit, CG6871 ELISA Kit, CT21282 ELISA Kit, CatA ELISA Kit, DMCATHPO ELISA Kit, DROCATHPO ELISA Kit, Dmel\\CG6871 ELISA Kit, U00145 ELISA Kit, bs36h11.y1 ELISA Kit, cat ELISA Kit, GB11648 ELISA Kit, catalase ELISA Kit, Cat ELISA Kit, BA0843 ELISA Kit, DKFZp469E0232 ELISA Kit, catalase ELISA Kit, Catalase ELISA Kit, catalase, gene 2 ELISA Kit, CAT ELISA Kit, Cat ELISA Kit, cat ELISA Kit, cat.2 ELISA Kit, LOC769804 ELISA Kit, BA_0843 ELISA Kit

Gen-ID: 847

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism