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ACP6 ELISA Kit

ACP6 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690814
  • Target Alle ACP6 ELISA Kits anzeigen
    ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human ACP6 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human ACP6.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle ACP6 ELISA Kits anzeigen
    ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))
    Andere Bezeichnung
    ACP6 (ACP6 Produkte)
    Synonyme
    ACP6 ELISA Kit, im:7147584 ELISA Kit, zgc:172268 ELISA Kit, MGC146066 ELISA Kit, ACPL1 ELISA Kit, LPAP ELISA Kit, PACPL1 ELISA Kit, 5730559A09Rik ELISA Kit, AU022842 ELISA Kit, mPACPL1 ELISA Kit, acid phosphatase 6, lysophosphatidic ELISA Kit, acid phosphatase 6, lysophosphatidic S homeolog ELISA Kit, ACP6 ELISA Kit, acp6 ELISA Kit, acp6.S ELISA Kit, Acp6 ELISA Kit
    Gen-ID
    51205
    UniProt
    Q9NPH0
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