SSB ELISA Kit

Produktnummer ABIN5674951

Produktdetails SSB ELISA Kit

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 29 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SSB

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Abstract: SSB Produkte

Synonyme: LARP3 ELISA Kit, La ELISA Kit, lab1 ELISA Kit, larp3 ELISA Kit, ssb ELISA Kit, Mt-SSB ELISA Kit, MtSSB ELISA Kit, Ssbp1 ELISA Kit, SS-B ELISA Kit, laa-a ELISA Kit, ssb-a ELISA Kit, ssb-b ELISA Kit, wu:fb17f11 ELISA Kit, zgc:55588 ELISA Kit, Sjogren syndrome antigen B ELISA Kit, Sjogren syndrome antigen B L homeolog ELISA Kit, single stranded DNA binding protein 1 ELISA Kit, Sjogren syndrome antigen B S homeolog ELISA Kit, Sjogren syndrome antigen B (autoantigen La) ELISA Kit, SSB ELISA Kit, ssb.L ELISA Kit, SSBP1 ELISA Kit, Ssb ELISA Kit, ssb.S ELISA Kit, ssb ELISA Kit

Hintergrund: Alternative name: LARP3, Lupus La Protein, Autoantigen La, La Ribonucleoprotein Domain Family,Member 3, Autoantigen SSB/La

UniProt: P38656