MYL3/CMLC1 ELISA Kit

Produktnummer ABIN5674946

Produktdetails MYL3/CMLC1 ELISA Kit

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0056 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Andere Bezeichnung: Myosin Light Chain 3 (MYL3) (MYL3 Produkte)

Synonyme: CMD1S ELISA Kit, CMH1 ELISA Kit, MPD1 ELISA Kit, MYHCB ELISA Kit, SPMD ELISA Kit, SPMM ELISA Kit, B-MHC ELISA Kit, MyHC-I ELISA Kit, Myhc-b ELISA Kit, Myhcb ELISA Kit, beta-MHC ELISA Kit, MLC1s ELISA Kit, MLC1v ELISA Kit, Mylc ELISA Kit, VLC1 ELISA Kit, Mylc1v ELISA Kit, CMH8 ELISA Kit, MLC1SB ELISA Kit, MLC1V ELISA Kit, mlc1v ELISA Kit, myl3-a ELISA Kit, myl3-b ELISA Kit, myl3.L ELISA Kit, zgc:103441 ELISA Kit, myosin heavy chain 7 ELISA Kit, myosin, heavy polypeptide 7, cardiac muscle, beta ELISA Kit, myosin, light polypeptide 3 ELISA Kit, myosin light chain 3 ELISA Kit, myosin light chain 3 S homeolog ELISA Kit, cardiac myosin light chain-1 ELISA Kit, MYH7 ELISA Kit, Myh7 ELISA Kit, Myl3 ELISA Kit, MYL3 ELISA Kit, myl3.S ELISA Kit, cmlc1 ELISA Kit

Hintergrund: Alternative name: CMH8, MLC1V, VLC1, MLC1SB, Cardiac myosin light chain 1, Ventricular/slow twitch myosin alkali light chain

Gen-ID: 24585

UniProt: P16409