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Parathymosin ELISA Kit

PTMS Reaktivität: Ratte Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Produktnummer ABIN5674936
  • Target Alle Parathymosin (PTMS) Produkte
    Parathymosin (PTMS)
    Reaktivität
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Sensitivität
    0.58 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle Parathymosin (PTMS) Produkte
    Parathymosin (PTMS)
    Abstract
    PTMS Produkte
    Synonyme
    ParaT ELISA Kit, ZnBP ELISA Kit, 2610009E16Rik ELISA Kit, parathymosin ELISA Kit, PTMS ELISA Kit, Ptms ELISA Kit
    Hintergrund
    Alternative name: ParaT
    Gen-ID
    83801
    UniProt
    P04550
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