Presenilin 2 ELISA Kit

Produktnummer ABIN5674924

Produktdetails Presenilin 2 ELISA Kit

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0106 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Presenilin 2

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Andere Bezeichnung: Presenilin 2 (PS2) (PSEN2 Produkte)

Synonyme: AD3L ELISA Kit, AD4 ELISA Kit, CMD1V ELISA Kit, PS2 ELISA Kit, STM2 ELISA Kit, ALG-3 ELISA Kit, Ad4h ELISA Kit, Alg3 ELISA Kit, PS-2 ELISA Kit, Psnl2 ELISA Kit, pre2 ELISA Kit, zf-PS2 ELISA Kit, PSNL2 ELISA Kit, PSEN2 ELISA Kit, ad4 ELISA Kit, ps2 ELISA Kit, ad3l ELISA Kit, stm2 ELISA Kit, Ps2 ELISA Kit, presenilin 2 ELISA Kit, presenilin 2 S homeolog ELISA Kit, presenilin 2 (Alzheimer disease 4) ELISA Kit, trefoil factor 1 ELISA Kit, PSEN2 ELISA Kit, Psen2 ELISA Kit, psen2 ELISA Kit, psen2.S ELISA Kit, Tff1 ELISA Kit

Hintergrund: Alternative name: PSEN2, AD3L, AD4, PS2, STM2, AD3LP, AD5, Alzheimer Disease 4

Gen-ID: 5664

UniProt: P49810

Pathways: Notch Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung