HMGCR ELISA Kit

Produktnummer ABIN5674904

Produktdetails HMGCR ELISA Kit

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.31 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Andere Bezeichnung: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) (HMGCR Produkte)

Synonyme: HMGR ELISA Kit, LDLCQ3 ELISA Kit, hmg-coa-r ELISA Kit, HMG-CoAR ELISA Kit, Red ELISA Kit, 3H3M ELISA Kit, BcDNA:LD15354 ELISA Kit, CG10367 ELISA Kit, Clb ELISA Kit, DmHMG-CoAr ELISA Kit, Dmel\\CG10367 ELISA Kit, HMGCR ELISA Kit, HMGCR_Dm ELISA Kit, HMGCoAR ELISA Kit, HMGCoARr ELISA Kit, HMGCoAr ELISA Kit, HmG-CoAR ELISA Kit, Hmg ELISA Kit, bro1 ELISA Kit, bs32f05.y1 ELISA Kit, clb ELISA Kit, hmgc ELISA Kit, hmgcr ELISA Kit, hmgrc ELISA Kit, l(3)01152 ELISA Kit, l(3)04684 ELISA Kit, hmgcr2 ELISA Kit, im:6912716 ELISA Kit, zgc:110746 ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog ELISA Kit, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit, HMG Coenzyme A reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase b ELISA Kit, HMGCR ELISA Kit, hmgcr.L ELISA Kit, Hmgcr ELISA Kit, hmgcrb ELISA Kit

Hintergrund: Alternative name: HMG-CoA Reductase, Hydroxymethylglutaryl-CoA Reductase

Gen-ID: 15357

UniProt: Q01237

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha