GOT2 ELISA Kit

Produktnummer ABIN5674877

Produktdetails GOT2 ELISA Kit

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0107 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Andere Bezeichnung: Aspartate Aminotransferase 2 (AST2) (GOT2 Produkte)

Synonyme: KAT4 ELISA Kit, KATIV ELISA Kit, mitAAT ELISA Kit, AI789014 ELISA Kit, Got-1 ELISA Kit, cAspAT ELISA Kit, cCAT ELISA Kit, AL022787 ELISA Kit, FABP-pm ELISA Kit, Got-2 ELISA Kit, mAspAT ELISA Kit, ASPATA ELISA Kit, got2 ELISA Kit, zgc:66329 ELISA Kit, fj40h07 ELISA Kit, wu:fj40h07 ELISA Kit, zgc:56425 ELISA Kit, glutamic-oxaloacetic transaminase 2 ELISA Kit, glutamic-oxaloacetic transaminase 1, soluble ELISA Kit, glutamatic-oxaloacetic transaminase 2, mitochondrial ELISA Kit, glutamic-oxaloacetic transaminase 2a, mitochondrial ELISA Kit, glutamic-oxaloacetic transaminase 2 L homeolog ELISA Kit, Aspartate amino transferase activity ELISA Kit, glutamic-oxaloacetic transaminase 2b, mitochondrial ELISA Kit, GOT2 ELISA Kit, got2 ELISA Kit, Got1 ELISA Kit, Got2 ELISA Kit, got2a ELISA Kit, got2.L ELISA Kit, AST ELISA Kit, got2b ELISA Kit

Hintergrund: Alternative name: GOT2, MitAAT, KATIV, KAT4, FABP-1, Plasma membrane-associated fatty acid-binding protein, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial, Kynurenine Aminotransferase IV

Gen-ID: 25721

UniProt: P00507

Pathways: Monocarboxylic Acid Catabolic Process