CPA1 ELISA Kit

Produktnummer ABIN5674872

Produktdetails CPA1 ELISA Kit

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 36 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Andere Bezeichnung: Carboxypeptidase A1, Pancreatic (CPA1) (CPA1 Produkte)

Synonyme: cpa1 ELISA Kit, cbpa1 ELISA Kit, CPA ELISA Kit, CPA1 ELISA Kit, 0910001L12Rik ELISA Kit, Cpa ELISA Kit, carboxypeptidase A1 ELISA Kit, pancreatic carboxypeptidase A1 ELISA Kit, Carboxypeptidase A1 ELISA Kit, carboxypeptidase A1-like ELISA Kit, carboxypeptidase a1 ELISA Kit, carboxypeptidase A1, pancreatic ELISA Kit, CpipJ_CPIJ007169 ELISA Kit, CpipJ_CPIJ010802 ELISA Kit, CpipJ_CPIJ010805 ELISA Kit, MCYG_06789 ELISA Kit, MGYG_03840 ELISA Kit, cpa1 ELISA Kit, cbpa1 ELISA Kit, LOC100224866 ELISA Kit, CPA1 ELISA Kit, Cpa1 ELISA Kit

Gen-ID: 109697

UniProt: Q7TPZ8