Aquaporin 1 ELISA Kit

Produktnummer ABIN5674869

Produktdetails Aquaporin 1 ELISA Kit

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0117 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Andere Bezeichnung: Aquaporin 1, Colton Blood Group (AQP1) (AQP1 Produkte)

Synonyme: fj33g04 ELISA Kit, zgc:85890 ELISA Kit, wu:fj33g04 ELISA Kit, AQP1 ELISA Kit, CHIP28 ELISA Kit, AQP-CHIP ELISA Kit, CO ELISA Kit, AQP-1 ELISA Kit, CHIP29 ELISA Kit, aquaporin 1a (Colton blood group), tandem duplicate 1 ELISA Kit, aquaporin 1 ELISA Kit, aquaporin 1 (Colton blood group) ELISA Kit, aqp1a.1 ELISA Kit, AQP1 ELISA Kit, Aqp1 ELISA Kit

Hintergrund: Alternative name: AQP-CHIP, CHIP28, CO, Channel-Forming Integral Protein,28 kDa, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Gen-ID: 25240

UniProt: P29975

Pathways: Hormone Transport