UQCRC2 ELISA Kit

Produktnummer ABIN5674841

Produktdetails UQCRC2 ELISA Kit

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0116 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu UQCRC2

Target: UQCRC2 (Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2))

Andere Bezeichnung: Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) (UQCRC2 Produkte)

Synonyme: MC3DN5 ELISA Kit, QCR2 ELISA Kit, UQCR2 ELISA Kit, fi06a11 ELISA Kit, uqcrc2 ELISA Kit, wu:fb64g04 ELISA Kit, wu:fi06a11 ELISA Kit, fb33d01 ELISA Kit, fc43c05 ELISA Kit, wu:fb33d01 ELISA Kit, wu:fc43c05 ELISA Kit, zgc:92453 ELISA Kit, MGC80228 ELISA Kit, MGC130698 ELISA Kit, Uqcrc2 ELISA Kit, Afu5g04210 ELISA Kit, AO090102000625 ELISA Kit, 1500004O06Rik ELISA Kit, UQCRC2 ELISA Kit, ubiquinol-cytochrome c reductase core protein 2 ELISA Kit, ubiquinol cytochrome c reductase core protein 2 ELISA Kit, ubiquinol-cytochrome c reductase core protein 2b ELISA Kit, ubiquinol-cytochrome c reductase core protein II ELISA Kit, ubiquinol-cytochrome c reductase core protein 2a ELISA Kit, ubiquinol-cytochrome c reductase core protein 2 L homeolog ELISA Kit, cytochrome b-c1 complex subunit 2, mitochondrial ELISA Kit, ubiquinol-cytochrome C reductase complex core protein 2 ELISA Kit, ubiquinol--cytochrome-c reductase subunit 2 ELISA Kit, UQCRC2 ELISA Kit, Uqcrc2 ELISA Kit, uqcrc2b ELISA Kit, uqcrc2a ELISA Kit, uqcrc2.L ELISA Kit, uqcrc2 ELISA Kit, LOC100026672 ELISA Kit, LOC100389097 ELISA Kit, LOC100456655 ELISA Kit, LOC100592631 ELISA Kit, AFUA_5G04210 ELISA Kit, ANI_1_872084 ELISA Kit, AOR_1_1048134 ELISA Kit, QCR2 ELISA Kit, LOC100861263 ELISA Kit

Hintergrund: Alternative name: QCR2, UQCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II

Gen-ID: 293448

UniProt: P32551