EGFL7 ELISA Kit

Produktnummer ABIN5674827

Produktdetails EGFL7 ELISA Kit

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 14.8 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu EGFL7

Target: EGFL7 (EGF-Like-Domain, Multiple 7 (EGFL7))

Andere Bezeichnung: EGF Like Domain Protein, Multiple 7 (EGFL7) (EGFL7 Produkte)

Synonyme: egfl7 ELISA Kit, MGC53105 ELISA Kit, EGFL7 ELISA Kit, zgc:136669 ELISA Kit, zgc:152804 ELISA Kit, zneu1 ELISA Kit, xegfl7 ELISA Kit, MGC147423 ELISA Kit, VE-statin ELISA Kit, Zneu1 ELISA Kit, NEU1 ELISA Kit, RP11-251M1.2 ELISA Kit, VE-STATIN ELISA Kit, ZNEU1 ELISA Kit, EGF like domain multiple 7 S homeolog ELISA Kit, EGF like domain multiple 7 ELISA Kit, EGF-like-domain, multiple 7 ELISA Kit, EGF-like domain 7 ELISA Kit, egfl7.S ELISA Kit, EGFL7 ELISA Kit, egfl7 ELISA Kit, Egfl7 ELISA Kit

Hintergrund: Alternative name: ZNEU1, MEGF7, Zneu1, Vascular endothelial statin, EGF Like Domain Containing 7, Epidermal growth factor-like protein 7, Multiple epidermal growth factor-like domains protein 7

Gen-ID: 51162

UniProt: Q9UHF1