DAB1 ELISA Kit

Produktnummer ABIN5674826

Produktdetails DAB1 ELISA Kit

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0146 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Andere Bezeichnung: Disabled Homolog 1 (DAB1) (DAB1 Produkte)

Synonyme: AI956902 ELISA Kit, C630028C02Rik ELISA Kit, scm ELISA Kit, scr ELISA Kit, scrambler ELISA Kit, yot ELISA Kit, disabled-1 ELISA Kit, LOC100232823 ELISA Kit, si:dkey-242m13.1 ELISA Kit, DAB1 ELISA Kit, zgc:136547 ELISA Kit, DAB1, reelin adaptor protein ELISA Kit, disabled 1 ELISA Kit, disabled homolog 1 ELISA Kit, Dab, reelin signal transducer, homolog 1a (Drosophila) ELISA Kit, Dab, reelin signal transducer, homolog 1b (Drosophila) ELISA Kit, DAB1 ELISA Kit, Dab1 ELISA Kit, LOC100232823 ELISA Kit, dab1a ELISA Kit, dab1b ELISA Kit

Gen-ID: 13131

UniProt: P97318