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UGCG ELISA Kit

UGCG Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674820
  • Target Alle UGCG ELISA Kits anzeigen
    UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))
    Reaktivität
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0115 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle UGCG ELISA Kits anzeigen
    UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))
    Andere Bezeichnung
    UDP Glucose Ceramide Glucosyltransferase (UGCG) (UGCG Produkte)
    Synonyme
    GCS ELISA Kit, GLCT1 ELISA Kit, XLCGT ELISA Kit, gcs ELISA Kit, glct1 ELISA Kit, ugcga ELISA Kit, xlcgt ELISA Kit, cb539 ELISA Kit, sb:cb539 ELISA Kit, zgc:112506 ELISA Kit, ugcg ELISA Kit, ugcgb ELISA Kit, AU043821 ELISA Kit, C80537 ELISA Kit, Epcs21 ELISA Kit, GlcT-1 ELISA Kit, Ugcgl ELISA Kit, UDP-glucose ceramide glucosyltransferase ELISA Kit, UDP-glucose ceramide glucosyltransferase L homeolog ELISA Kit, UDP-glucose ceramide glucosyltransferase S homeolog ELISA Kit, UGCG ELISA Kit, ugcg.L ELISA Kit, Ugcg ELISA Kit, ugcg ELISA Kit, ugcg.S ELISA Kit
    Hintergrund
    Alternative name: GCS, GLCT-1, Glucosylceramide Synthase, N-acylsphingosine D-glucosyltransferase
    Gen-ID
    7357
    UniProt
    Q16739
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