ALDOC ELISA Kit

Produktnummer ABIN5674806

Produktdetails ALDOC ELISA Kit

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 0278 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ALDOC

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Andere Bezeichnung: Aldolase C, Fructose Bisphosphate (ALDOC) (ALDOC Produkte)

Synonyme: ALDC ELISA Kit, MGC69434 ELISA Kit, ALDOC ELISA Kit, aldoc ELISA Kit, AI847350 ELISA Kit, AU040929 ELISA Kit, Aldo3 ELISA Kit, Scrg2 ELISA Kit, ALDCAA ELISA Kit, F16dip7 ELISA Kit, RATALDCAA ELISA Kit, aldocl ELISA Kit, wu:fj56h04 ELISA Kit, zgc:112357 ELISA Kit, QccE-21970 ELISA Kit, aldolase, fructose-bisphosphate C ELISA Kit, aldolase, fructose-bisphosphate C L homeolog ELISA Kit, aldolase C, fructose-bisphosphate, b ELISA Kit, aldolase C, fructose-bisphosphate ELISA Kit, aldolase A, fructose-bisphosphate ELISA Kit, aldolase C, fructose-bisphosphate, a ELISA Kit, Fructose-bisphosphate aldolase C ELISA Kit, ALDOC ELISA Kit, aldoc.L ELISA Kit, aldoc ELISA Kit, aldocb ELISA Kit, Aldoc ELISA Kit, ALDOA ELISA Kit, aldoca ELISA Kit

Hintergrund: Alternative name: ALDO-C, ALDC, ALD-C, Fructose-bisphosphate aldolase C, Brain-type aldolase

Gen-ID: 11676

UniProt: P05063