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Utrophin ELISA Kit

UTRN Reaktivität: Human Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674792
  • Target Alle Utrophin (UTRN) ELISA Kits anzeigen
    Utrophin (UTRN)
    Reaktivität
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.625 ng/mL - 40 ng/mL
    Untere Nachweisgrenze
    0.625 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0252 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle Utrophin (UTRN) ELISA Kits anzeigen
    Utrophin (UTRN)
    Abstract
    UTRN Produkte
    Synonyme
    drp ELISA Kit, dmdl ELISA Kit, drp1 ELISA Kit, DKFZp469A0710 ELISA Kit, DMDL ELISA Kit, DRP ELISA Kit, DRP1 ELISA Kit, AA589569 ELISA Kit, Dmdl ELISA Kit, utrophin L homeolog ELISA Kit, utrophin ELISA Kit, utrn.L ELISA Kit, UTRN ELISA Kit, LOAG_03796 ELISA Kit, utrn ELISA Kit, LOC100551145 ELISA Kit, Utrn ELISA Kit
    Hintergrund
    Alternative name: DRP1, DMDL, DRP, Homologous To Dystrophin, Dystrophin-related protein 1
    Gen-ID
    7402
    UniProt
    P46939
    Pathways
    Skeletal Muscle Fiber Development
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