CAT2 ELISA Kit
CAT2
Reaktivität: Maus
Colorimetric
Sandwich ELISA
0.312 ng/mL - 20 ng/mL
Tissue Homogenate
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- Target Alle CAT2 Produkte
- CAT2 (Cationic Amino Acid Transporter 2 (CAT2))
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Sensitivität
- 0124 ng/mL
- Bestandteile
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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Cationic Amino Acid Transporter 2 (CAT2) ELISA Kit
CAT2 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdauer
- 1 - 4.5 h
- Plattentyp
- Pre-coated
- Protokoll
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
-
- Target Alle CAT2 Produkte
- CAT2 (Cationic Amino Acid Transporter 2 (CAT2))
- Abstract
- CAT2 Produkte
- Synonyme
- cationic amino acid transporter 2 ELISA Kit, cationic amino acid transporter 2 ELISA Kit, GIT ArfGAP 2 ELISA Kit, CAT2 ELISA Kit, Git2 ELISA Kit
- Hintergrund
- Alternative name: SLC7A2, ATRC2, CAT-2, HCAT2, Low Affinity Cationic Amino Acid Transporter 2, Solute Carrier Family 7 Member 2,Cationic Amino Acid Transporter,y+ System
- Gen-ID
- 11988
- UniProt
- P18581
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