SIRT2 ELISA Kit

Produktnummer ABIN5674759

Produktdetails SIRT2 ELISA Kit

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.61 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Abstract: SIRT2 Produkte

Synonyme: CG5085 ELISA Kit, D.mel2 ELISA Kit, Dmel\\CG5085 ELISA Kit, SIRT2 ELISA Kit, dSIRT2 ELISA Kit, sir2 ELISA Kit, Sirt2 ELISA Kit, BmSirt2 ELISA Kit, AtSRT2 ELISA Kit, SIRTUIN 2 ELISA Kit, T2K12.8 ELISA Kit, sirtuin 2 ELISA Kit, SIR2 ELISA Kit, SIR2L ELISA Kit, SIR2L2 ELISA Kit, 5730427M03Rik ELISA Kit, Sir2l ELISA Kit, wu:fb57d05 ELISA Kit, zgc:55966 ELISA Kit, zgc:77003 ELISA Kit, Sirtuin 2 ELISA Kit, sirtuin 2 ELISA Kit, sirtuin 2 S homeolog ELISA Kit, NAD-dependent deacetylase sirtuin 2 homolog ELISA Kit, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) ELISA Kit, Sirt2 ELISA Kit, SIRT2 ELISA Kit, sirt2.S ELISA Kit, SRT2 ELISA Kit, sirt2 ELISA Kit

Hintergrund: Alternative name: SIR2L, SIR2-L, SIR2L2, Silent Information Regulator 2 Protein, NAD-Dependent Deacetylase Sirtuin-2, Silent Mating Type Information Regulation 2 Homolog

Gen-ID: 64383

UniProt: Q8VDQ8