SCARA3 ELISA Kit

Produktnummer ABIN5674745

Produktdetails SCARA3 ELISA Kit

Target: SCARA3 (Scavenger Receptor Class A, Member 3 (SCARA3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.34 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SCARA3

Target: SCARA3 (Scavenger Receptor Class A, Member 3 (SCARA3))

Andere Bezeichnung: Scavenger Receptor Class A Member 3 (SCARA3) (SCARA3 Produkte)

Synonyme: APC7 ELISA Kit, CSR ELISA Kit, CSR1 ELISA Kit, MSLR1 ELISA Kit, MSRL1 ELISA Kit, SCARA3 ELISA Kit, C130058N24Rik ELISA Kit, scavenger receptor class A member 3 ELISA Kit, scavenger receptor class A, member 3 ELISA Kit, SCARA3 ELISA Kit, Scara3 ELISA Kit, scara3 ELISA Kit

Hintergrund: Alternative name: CSR1, CSR, MSLR1, APC7, MSRL1, Macrophage Scavenger Receptor-Like 1, Cellular stress response gene protein

Gen-ID: 51435

UniProt: Q6AZY7