LCT ELISA Kit
LCT
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Tissue Homogenate
-
- Target Alle LCT ELISA Kits anzeigen
- LCT (Lactase (LCT))
-
Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Sensitivität
- 0.05 ng/mL
- Bestandteile
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Lactase (LCT) ELISA Kit
LCT Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
-
- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdauer
- 1 - 4.5 h
- Plattentyp
- Pre-coated
- Protokoll
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
-
- Target Alle LCT ELISA Kits anzeigen
- LCT (Lactase (LCT))
- Abstract
- LCT Produkte
- Synonyme
- LAC ELISA Kit, LPH ELISA Kit, LPH1 ELISA Kit, Lph ELISA Kit, Gm100 ELISA Kit, Lphl ELISA Kit, LCT ELISA Kit, PNGH ELISA Kit, GLH1 ELISA Kit, lac ELISA Kit, lct ELISA Kit, lph ELISA Kit, lph1 ELISA Kit, lactase ELISA Kit, lactase ELISA Kit, lactase, gene 1 L homeolog ELISA Kit, lactase, gene 2 L homeolog ELISA Kit, lactase, gene 2 ELISA Kit, lactase phlorizin hydrolase ELISA Kit, LCT ELISA Kit, Lct ELISA Kit, lct.1.L ELISA Kit, lct.2.L ELISA Kit, lct.2 ELISA Kit, LPH ELISA Kit
- Hintergrund
- Alternative name: LAC, LPH, LPH1, Lactose-Phlorizin Hydrolase
- Gen-ID
- 3938
- UniProt
- P09848
-
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