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TUBB1 ELISA Kit

TUBB1 Reaktivität: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674731
  • Target Alle TUBB1 ELISA Kits anzeigen
    TUBB1 (Tubulin, beta 1 (TUBB1))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.125 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.125 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    30.8 pg/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle TUBB1 ELISA Kits anzeigen
    TUBB1 (Tubulin, beta 1 (TUBB1))
    Andere Bezeichnung
    Tubulin Beta 1 (TUBb1) (TUBB1 Produkte)
    Synonyme
    TUBB3 ELISA Kit, 2810484G07Rik ELISA Kit, M(beta)1 ELISA Kit, TUBB1 ELISA Kit, tubulin beta 1 class VI ELISA Kit, tubulin, beta 1 class VI ELISA Kit, TUBB1 ELISA Kit, Tubb1 ELISA Kit
    Gen-ID
    81027
    UniProt
    Q9H4B7
    Pathways
    Microtubule Dynamics
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