SREBF1 ELISA Kit

Produktnummer ABIN5674726

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte SREBF1.

Kurzübersicht für SREBF1 ELISA Kit (ABIN5674726)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.39 ng/mL - 25 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails SREBF1 ELISA Kit

Untere Nachweisgrenze: 0.39 ng/mL

Analytische Methode: Quantitative

Sensitivität: 0144 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Hintergrund: Alternative name: SREBP1, Sterol Regulatory Element-Binding Protein 1, Adipocyte determination- and differentiation-dependent factor 1, ADD1

Gen-ID: 78968

UniProt: P56720

Pathways: AMPK Signaling, Caspase Kaskade in der Apoptose, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha