Nucleobindin 1 ELISA Kit

Produktnummer ABIN5674716

Produktdetails Nucleobindin 1 ELISA Kit

Target: Nucleobindin 1 (NUCB1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0068 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Nucleobindin 1

Target: Nucleobindin 1 (NUCB1)

Abstract: NUCB1 Produkte

Synonyme: nuc ELISA Kit, MGC69294 ELISA Kit, MGC81496 ELISA Kit, NUCB1 ELISA Kit, zgc:153192 ELISA Kit, DKFZp459O1814 ELISA Kit, B230337F23Rik ELISA Kit, C77483 ELISA Kit, Calnuc ELISA Kit, MTEST82 ELISA Kit, Nucb ELISA Kit, CALNUC ELISA Kit, NUC ELISA Kit, nucleobindin 1 ELISA Kit, nucleobindin 1 S homeolog ELISA Kit, nucb1 ELISA Kit, nucb1.S ELISA Kit, NUCB1 ELISA Kit, Nucb1 ELISA Kit

Hintergrund: Alternative name: NUC, CALNUC

Gen-ID: 4924

UniProt: Q02818