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NCDN ELISA Kit

NCDN Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5674712
  • Target Alle NCDN ELISA Kits anzeigen
    NCDN (Neurochondrin (NCDN))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0061 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle NCDN ELISA Kits anzeigen
    NCDN (Neurochondrin (NCDN))
    Abstract
    NCDN Produkte
    Synonyme
    CG2330 ELISA Kit, Dmel\\CG2330 ELISA Kit, NCDN ELISA Kit, GB14786 ELISA Kit, AU042419 ELISA Kit, MMS10-AE ELISA Kit, Ms10ae ELISA Kit, mKIAA0607 ELISA Kit, norbin ELISA Kit, Norbin ELISA Kit, CG2330 gene product from transcript CG2330-RA ELISA Kit, neurochondrin ELISA Kit, N-acylneuraminate-9-phosphatase ELISA Kit, neurochondrin S homeolog ELISA Kit, Neurochondrin ELISA Kit, NCDN ELISA Kit, LOC552430 ELISA Kit, ncdn ELISA Kit, Ncdn ELISA Kit, ncdn.S ELISA Kit
    Hintergrund
    Alternative name: Norbin
    Gen-ID
    23154
    UniProt
    Q9UBB6
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