NRF1 ELISA Kit

Produktnummer ABIN5674707

Produktdetails NRF1 ELISA Kit

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 29.7 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Abstract: NRF1 Produkte

Synonyme: ALPHA-PAL ELISA Kit, C87038 ELISA Kit, D6Ertd415e ELISA Kit, SC:BZ3C13.1 ELISA Kit, SC:bZ3C13.2 ELISA Kit, ube2h ELISA Kit, Nfe2l1_retired ELISA Kit, IBR/F ELISA Kit, NRF1 ELISA Kit, alpha-pal ELISA Kit, nrf-1 ELISA Kit, LOC100220087 ELISA Kit, nuclear respiratory factor 1 ELISA Kit, nuclear respiratory factor 1 S homeolog ELISA Kit, NRF1 ELISA Kit, Nrf1 ELISA Kit, nrf1 ELISA Kit, nrf1.S ELISA Kit

Hintergrund: Alternative name: ALPHA-PAL, EWG, Alpha Palindromic-Binding Protein

Gen-ID: 18181

UniProt: Q9WU00

Pathways: Regulation of Lipid Metabolism by PPARalpha