NKAP ELISA Kit
Kurzübersicht für NKAP ELISA Kit (ABIN5674704)
Target
Alle NKAP ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 31.25 pg/mL
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Analytische Methode
- Quantitative
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Sensitivität
- 11.3 pg/mL
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Bestandteile
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual -
Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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NFKB Activating Protein (NKAP) ELISA Kit
NKAP Reaktivität: Ratte Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
NFKB Activating Protein (NKAP) ELISA KitNKAP Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
NFKB Activating Protein (NKAP) ELISA KitNKAP Reaktivität: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
NFKB Activating Protein (NKAP) ELISA KitNKAP Reaktivität: Ratte Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Testdauer
- 1 - 4.5 h
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Plattentyp
- Pre-coated
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Protokoll
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Haltbarkeit
- 12 months
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- NKAP (NFKB Activating Protein (NKAP))
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Hintergrund
- Alternative name: NF Kappa B Activating Protein
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Gen-ID
- 67050
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UniProt
- Q9D0F4
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Pathways
- Stem Cell Maintenance, Phosphorylierungen bei SARS-CoV-2 Infektion
Target Alle NKAP ELISA Kits anzeigen
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