HRH3 ELISA Kit
HRH3
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Tissue Homogenate
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- Target Alle HRH3 ELISA Kits anzeigen
- HRH3 (Histamine Receptor H3 (HRH3))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Sensitivität
- 0053 ng/mL
- Bestandteile
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product HRH3 ELISA Kit
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Histamine Receptor H3 (HRH3) ELISA Kit
HRH3 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdauer
- 1 - 4.5 h
- Plattentyp
- Pre-coated
- Protokoll
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target Alle HRH3 ELISA Kits anzeigen
- HRH3 (Histamine Receptor H3 (HRH3))
- Abstract
- HRH3 Produkte
- Synonyme
- GPCR97 ELISA Kit, HH3R ELISA Kit, AW049250 ELISA Kit, H3R ELISA Kit, histamine receptor H3 ELISA Kit, HRH3 ELISA Kit, Hrh3 ELISA Kit
- Hintergrund
- Alternative name: GPCR97, HH3R, G-protein coupled receptor 97
- Gen-ID
- 11255
- UniProt
- Q9Y5N1
- Pathways
- cAMP Metabolic Process, Feeding Behaviour
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