Apelin Receptor ELISA Kit

Produktnummer ABIN5674667

Produktdetails Apelin Receptor ELISA Kit

Target: Apelin Receptor (APLNR)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.625 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.625 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 5.9 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Apelin Receptor

Target: Apelin Receptor (APLNR)

Abstract: APLNR Produkte

Synonyme: AGTRL1 ELISA Kit, APJ ELISA Kit, APJR ELISA Kit, HG11 ELISA Kit, Agtrl1 ELISA Kit, msr/apj ELISA Kit, Apj ELISA Kit, X-msr ELISA Kit, Xangio1 ELISA Kit, Xmsr ELISA Kit, agtrl1 ELISA Kit, angio1 ELISA Kit, apj ELISA Kit, apjr ELISA Kit, hg11 ELISA Kit, msr ELISA Kit, APLNR ELISA Kit, DKFZp469C1532 ELISA Kit, agtrl1-B ELISA Kit, agtrl1b ELISA Kit, Agtrl1a ELISA Kit, grn ELISA Kit, zgc:114063 ELISA Kit, APJa ELISA Kit, XAngio1 ELISA Kit, agtrl1-A ELISA Kit, agtrl1a ELISA Kit, aplnr-a ELISA Kit, aplnr-b ELISA Kit, x-MSR ELISA Kit, xAngio1 ELISA Kit, APLNR1 ELISA Kit, apelin receptor ELISA Kit, apelin receptor S homeolog ELISA Kit, apelin receptor b ELISA Kit, apelin receptor L homeolog ELISA Kit, APLNR ELISA Kit, Aplnr ELISA Kit, aplnr ELISA Kit, aplnr.S ELISA Kit, aplnrb ELISA Kit, aplnr.L ELISA Kit

Hintergrund: Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1

Gen-ID: 23796

UniProt: Q9WV08