ZG16B CLIA Kit

Produktnummer ABIN5660085

Dieses Chemiluminescent ELISA-Kit wurde entwickelt für die quantitative Messung von Human ZG16B.

Kurzübersicht für ZG16B CLIA Kit (ABIN5660085)

Target: ZG16B (Zymogen Granule Protein 16 Homolog B (ZG16B))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 312.5 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails ZG16B CLIA Kit

Untere Nachweisgrenze: 312.5 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Zymogen Granule Protein 16 Homolog B (ZG16B). No significant cross-reactivity or interference between Zymogen Granule Protein 16 Homolog B (ZG16B) and analogues was observed.

Sensitivität: 11.16 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zymogen Granule Protein 16 Homolog B (ZG16B) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: ZG16B (Zymogen Granule Protein 16 Homolog B (ZG16B))

Andere Bezeichnung: Zymogen Granule Protein 16 Homolog B

Hintergrund:

Gene Name: Zymogen Granule Protein 16 Homolog B

Gene Aliases: JCLN2, Jacalin-Like Lectin Domain Containing 2

Gen-ID: 124220

UniProt: Q96DA0